Photoshop, flash, fireworks, mattes in image effects graphics (Need quick advice)

[NappyXD]

PNGs support the alpha channel that you're looking for. Save in PNG format.

Works and with 10% of the .tif file size, thanks.

Edit:

if whatever you're eventually going to be putting the effect in supports APNG, save it as that. I'll keep the transparency from PNG, yet have the animation properties of a GIF.

Thank you, I'll mess around with that.