Can I make .png files with programming also what do you use tsr sprites

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Yea, sure. Generally, every programming language that can write files should be suitable. However, the PNG format is hilariously complicated, so you'd better use one that has finished libraries available. C# has what you need, I bet Java would have them too, C++ has anything somewhere on the internet, there's libraries for Flash AS3. For Delphi Pascal, I use pngdelphi, also found on the internet.
Theoretically, you could also write your own code, but that is far from easy, so just search the internet for available libraries, classes or source code to load/save PNG from whatever programming language you want to use.